QIAquick Gel Extraction Kit

Component	Volume (ul)	Mix (ul)
Forward primer
Reversed primer
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)
ddH₂O
Total volume

Forward primer(s):

LCO1490

Specimens:

SMNH UJ 10-254

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1
Amplification		Z	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-
Annealing	^{The primer binds to the template}	-
Extension	^{The DNA polymerase synthesizes new DNA}	-
Final elongation	^{Any remaining single-stranded DNA is extended}	1
Final hold	^{Short-term storage}	1	-

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

HCO2198

Gel Extraction 4FB/1806R 20110131

Date:

Wed, 2011-01-26 00:00

Gene:

18S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer
Reversed primer
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)
ddH₂O
Total volume

Forward primer(s):

4FB

Specimens:

SMNH UJ 10-250

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1
Amplification		Z	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-
Annealing	^{The primer binds to the template}	-
Extension	^{The DNA polymerase synthesizes new DNA}	-
Final elongation	^{Any remaining single-stranded DNA is extended}	1
Final hold	^{Short-term storage}	1	-

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

1806R

Gel Extraction S30/5FK 20101130

Date:

Mon, 2010-10-11 23:00

Gene:

18S

Template:

dna

PCR cocktail:

Forward primer(s):

Specimens:

Thermal cycling:

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

5FK

Gel Extraction 4FB/1806R 20110126

Date:

Tue, 2011-01-25 00:00

Gene:

18S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer
Reversed primer
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)
ddH₂O
Total volume

Forward primer(s):

Specimens:

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1
Amplification		Z	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-
Annealing	^{The primer binds to the template}	-
Extension	^{The DNA polymerase synthesizes new DNA}	-
Final elongation	^{Any remaining single-stranded DNA is extended}	1
Final hold	^{Short-term storage}	1	-

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

1806R

Gel Extraction S30/5FK 20110126

Date:

Tue, 2011-01-25 00:00

Gene:

18S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer
Reversed primer
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)
ddH₂O
Total volume

Forward primer(s):

S30

Specimens:

SMNH UJ 10-250

SMNH UJ 10-251

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1
Amplification		Z	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-
Annealing	^{The primer binds to the template}	-
Extension	^{The DNA polymerase synthesizes new DNA}	-
Final elongation	^{Any remaining single-stranded DNA is extended}	1
Final hold	^{Short-term storage}	1	-

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

5FK

Gel Extraction UJ2176/3449L 20110131

Date:

Mon, 2011-01-31 00:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer
Reversed primer
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)
ddH₂O
Total volume

Forward primer(s):

UJ2176

Specimens:

SMNH UJ 10-254

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1
Amplification		Z	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-
Annealing	^{The primer binds to the template}	-
Extension	^{The DNA polymerase synthesizes new DNA}	-
Final elongation	^{Any remaining single-stranded DNA is extended}	1
Final hold	^{Short-term storage}	1	-

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

L3449

Gel Extraction UJ2176/3449L 20110517

Date:

Mon, 2011-05-16 23:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer
Reversed primer
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)
ddH₂O
Total volume

Forward primer(s):

Specimens:

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1
Amplification		Z	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-
Annealing	^{The primer binds to the template}	-
Extension	^{The DNA polymerase synthesizes new DNA}	-
Final elongation	^{Any remaining single-stranded DNA is extended}	1
Final hold	^{Short-term storage}	1	-

Notes:

20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.

Reversed primer(s):

L3449

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QIAquick Gel Extraction Kit

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Gel Extraction S30/5FK 20110126

Gel Extraction UJ2176/3449L 20110131

Gel Extraction UJ2176/3449L 20110517

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QIAquick Gel Extraction Kit

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