COI- LCO/HCO 20100901

Procedure

PCR cocktail: 
Component Volume (ul) Mix (ul)
Forward primer  0.5  
Reversed primer  0.5  
Buffer    
MgCl2    
dNTPs    
Taq    
DNA (if nested)  2  
ddH2O
 22  
Total volume  25
Forward primer(s): 
Thermal cycling (abbreviated): 
95ºC@4min, 44x(94ºC@30sec, 46ºC@30sec, 72ºC@30sec), 72ºC@10min
Thermal cycling: 
Step Comment Cycles Time (sec) Temperature (°C)
Initialization For DNA polymerases that need hot-start heat activation 1 240
95  
Denaturation DNA melting producing single-stranded DNA      
Amplification   44 -  -
Denaturation DNA melting producing single-stranded DNA
 - 30 94 
Annealing The primer binds to the template  - 30  46
Extension The DNA polymerase synthesizes new DNA  - 30  72
Final elongation Any remaining single-stranded DNA is extended 1 600  72
Final hold Short-term storage 1 - 12 
Notes: 
10-22-2 extraction from worm no. 2 in the same sample tube.
PCR program: 
COI
Reversed primer(s): 
File attachments: 
Products and Equipment: 

Add new comment

You must have Javascript enabled to use this form.
Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith