CytB- Cytb-InoF/Cytb-InoR 20120416

Procedure

PCR cocktail: 
Component Volume (ul) Mix (ul)
Forward primer  1  
Reversed primer  1  
Buffer    
MgCl2    
dNTPs    
Taq    
DNA  2  
ddH2O
 22  
Total volume  26
Forward primer(s): 
Thermal cycling (abbreviated): 
95ºC@4min, 39x(94ºC@30sec, 49-71ºC@5sec, 72ºC@20sec), 72ºC@10min
Thermal cycling: 
Step Comment Cycles Time (sec) Temperature (°C)
Initialization For DNA polymerases that need hot-start heat activation  1 240  95 
Denaturation DNA melting producing single-stranded DNA  
 
Amplification   39  -  -
Denaturation DNA melting producing single-stranded DNA
 - 30 94 
Annealing The primer binds to the template  - 49-71 
Extension The DNA polymerase synthesizes new DNA  - 20   72
Final elongation Any remaining single-stranded DNA is extended  1 600  72 
Final hold Short-term storage  1 -  12
Reversed primer(s): 
File attachments: 
People: 
Products and Equipment: 

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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith