Basic information
Date:
Wed, 2008-10-01 23:00
Gene:
Template:
dna
Specimens:
Procedure
PCR cocktail:
Component | Volume (ul) | Mix (ul) |
Forward primer | 0.5 | |
Reversed primer | 0.5 | |
Buffer | ||
MgCl2 | ||
dNTPs | ||
Taq | ||
DNA (if nested) | 2 | |
ddH2O |
22 | |
Total volume | 25 |
Forward primer(s):
Thermal cycling (abbreviated):
95ºC@4min, 40x(94ºC@30sec, 52ºC@30sec, 72ºC@30sec), 72ºC@10min
PCR machine:
GeneAmp
Thermal cycling:
Step | Comment | Cycles | Time (sec) | Temperature (°C) |
Initialization | For DNA polymerases that need hot-start heat activation | 1 | ||
Denaturation | DNA melting producing single-stranded DNA | 1 | 240 | 95 |
Amplification | 40 | |||
Denaturation | 30 | 94 | ||
Annealing | 30 | 52 | ||
Extension | 30 | 72 | ||
Final elongation | Any remaining single-stranded DNA is extended | 1 | 600 | 72 |
Final hold | Short-term storage | 1 | - | 12 |
Notes:
2 worms were extracted separately from sample tube UJ08-119. UJ08-119 (no. 11 ) first extraction. UJ08-119-alt (no.12) second extraction.
PCR program:
S30
Reversed primer(s):
File attachments:
People:
Products and Equipment:
Add new comment