The Stylet

Diversity and Systematics of Acoela and Nemertodermatida

Gel Extraction UJ2176/3449L 20110517

Basic information

Date: 
Mon, 2011-05-16 22:00
Gene: 
Template: 
dna

Procedure

PCR cocktail: 
Component Volume (ul) Mix (ul)
Forward primer    
Reversed primer    
Buffer    
MgCl2    
dNTPs    
Taq    
DNA (if nested)    
ddH2O
   
Total volume  
Forward primer(s): 
Thermal cycling: 
Step Comment Cycles Time (sec) Temperature (°C)
Initialization For DNA polymerases that need hot-start heat activation 1
 
Denaturation DNA melting producing single-stranded DNA 1
 
Amplification   Z -  -
Denaturation DNA melting producing single-stranded DNA
 -
 
Annealing The primer binds to the template  -
 
Extension The DNA polymerase synthesizes new DNA  -
 
Final elongation Any remaining single-stranded DNA is extended 1
 
Final hold Short-term storage 1 -  
Notes: 
20 ul PCR-product was Gel Extracted and eluted in 35ul EB according to the QIAquick Gel Extraction Kit spin protocol.
Reversed primer(s): 
File attachments: 
Products and Equipment: 

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Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith