Andreas Wallberg

Classification of Acoela

The classification of Acoela down to the “family level” was recently revised based on a phylogenetic study (U. Jondelius, A. Wallberg, M. Hooge, and O. I. Raikova, “How the Worm Got its Pharynx: Phylogeny, Classification and Bayesian assessment of Character Evolution in Acoela,” Systematic Biology, 2011.doi:10.1093/sysbio/syr073 )

Lab Members & Current Projects

The Stylet is currently under construction.

Gel Cleaning 28S- 2093F/3449L 20080212

Date:

Tue, 2008-02-12 00:00

Gene:

28S

Template:

dna

Associated reaction(s):

28S- 2093F/3449L 20080206

PCR cocktail:

Forward primer(s):

F2093

Specimens:

SMNH UJ 07-55

SMNH - UJ - 07-72

PCR machine:

GeneAmp

Thermal cycling:

Step

Comment

Cycles

Time (sec)

Temperature (°C)

Reversed primer(s):

L3449

28S- 2093F/3449L 20080206

Date:

Wed, 2008-02-06 00:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	0,5
Reversed primer	0,5
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	2
ddH₂O	22
Total volume

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@5min, 2x(94ºC@30sec, 58-54ºC@30sec,72ºC@30), 34x(94ºC@30sec, 52ºC@30sec, 72ºC@60sec),72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1	300	95
Amplification		2	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-	30	94
Annealing	^{The primer binds to the template}	-	30	58-54
Extension	^{The DNA polymerase synthesizes new DNA}	-	30	72
Amplification		34
Denaturation			30	94
Annealing			30	52
Extension			60	72
Final elongation	^{Any remaining single-stranded DNA is extended}		600	72
Final hold	^{Short-term storage}	1	-	12

Reversed primer(s):

L3449

18S-S30/5FK 20080109

Date:

Wed, 2008-01-09 00:00

Template:

dna

Associated reaction(s):

18S-4FB/1806R 20080110

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	0.5
Reversed primer	0.5
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	2
ddH₂O	22
Total volume	25

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@5min, 40X(94ºC@30sec,52ºC@30sec, 72ºC@30sec)72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1	300	95
Amplification		40
Denaturation	^{DNA melting producing single-stranded DNA}		30	94
Annealing	^{The primer binds to the template}		30	52
Extension	^{The primer binds to the template}		30	72
Final elongation	^{Any remaining single-stranded DNA is extended}	1	600	72
Hold		1		8

Reversed primer(s):

5FK

COI- LCO/HCO 20071105

Date:

Mon, 2007-11-05 00:00

Gene:

COI

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	1
Reversed primer	1
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	1
ddH₂O	22
Total volume	25

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@5min, 40x(94ºC@15sec,46ºC@15sec, 72ºC@15sec),72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1	300	95
Amplification		40
Denaturation			15	94
Annealing			15	46
Extension			15	72
Final elongation	^{Any remaining single-stranded DNA is extended}	1	600	72
Hold		1		10

Reversed primer(s):

HCO2198

28S- 1200F/2450R 20071119

Date:

Mon, 2007-11-19 00:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	0.5
Reversed primer	0.5
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	1
ddH₂O	22
Total volume	24

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@5min, 40x(94ºC@30sec,52ºC@30sec, 72ºC@60sec),72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}
Denaturation	^{DNA melting producing single-stranded DNA}	1	300	95
Amplification		40
Denaturation			30	94
Annealing			30	52
Extension			60	72
Final elongation	^{Any remaining single-stranded DNA is extended}	1	600	72
Hold		1		8

Reversed primer(s):

2450R

28S- 2093F/3449L 20071107

Date:

Wed, 2007-11-07 00:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	0,5
Reversed primer	0,5
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	1
ddH₂O	23
Total volume

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@4min, 2x(94ºC@30sec, 58-54ºC@30sec,72ºC@30), 34x(94ºC@30sec, 52ºC@30sec, 72ºC@60sec),72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}
Denaturation	^{DNA melting producing single-stranded DNA}	1	240	95
Amplification		1	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-	30	94
Annealing	^{The primer binds to the template}	-	30	58-54
Extension	^{The DNA polymerase synthesizes new DNA}	-	30	72
Amplification		34
Denaturation			30	94
Annealing			30	52
Extension			60	72
Final elongation	^{Any remaining single-stranded DNA is extended}		600	72
Final hold	^{Short-term storage}	1	-	12

Reversed primer(s):

L3449

28S- 1200F/2450R 20071107

Date:

Wed, 2007-11-07 00:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	0.5
Reversed primer	0.5
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	1
ddH₂O	22
Total volume	24

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@5min, 40x(94ºC@30sec,52ºC@30sec, 72ºC@60sec),72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}
Denaturation	^{DNA melting producing single-stranded DNA}	1	300	95
Amplification		40
Denaturation			30	94
Annealing			30	52
Extension			60	72
Final elongation	^{Any remaining single-stranded DNA is extended}	1	600	72
Hold		1		8

Reversed primer(s):

2450R

28S-U178/L1642 20071106

Date:

Tue, 2007-11-06 00:00

Gene:

28S

Template:

dna

PCR cocktail:

Component	Volume (ul)	Mix (ul)
Forward primer	1
Reversed primer	1
Buffer
MgCl₂
dNTPs
Taq
DNA (if nested)	1
ddH₂O	22
Total volume	25

Forward primer(s):

Specimens:

Thermal cycling (abbreviated):

95ºC@5min, 2x(94ºC@30sec, 55-54ºC@30sec,72ºC@30), 34x(94ºC@30sec, 52ºC@30sec, 72ºC@60sec),72ºC@10min

PCR machine:

GeneAmp

Thermal cycling:

Step	Comment	Cycles	Time (sec)	Temperature (°C)
Initialization	^{For DNA polymerases that need hot-start heat activation}	1
Denaturation	^{DNA melting producing single-stranded DNA}	1	300	95
Amplification		2	-	-
Denaturation	^{DNA melting producing single-stranded DNA}	-	30	94
Annealing	^{The primer binds to the template}	-	30	55-54
Extension	^{The DNA polymerase synthesizes new DNA}	-	30	72
Amplification		34
Denaturation			30	94
Annealing			30	52
Extension			60	72
Final elongation	^{Any remaining single-stranded DNA is extended}		600	72
Final hold	^{Short-term storage}	1	-	10

Reversed primer(s):

L1642

The Stylet

Diversity and Systematics of Acoela and Nemertodermatida

People

Classification of Acoela

Lab Members & Current Projects

Gel Cleaning 28S- 2093F/3449L 20080212

28S- 2093F/3449L 20080206

18S-S30/5FK 20080109

COI- LCO/HCO 20071105

28S- 1200F/2450R 20071119

28S- 2093F/3449L 20071107

28S- 1200F/2450R 20071107

28S-U178/L1642 20071106

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Andreas Wallberg

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